超低分子量蛋白質(zhì)Marker III（3.4-100kD）

Ultra Low Molecular Weight Protein Marker III

●產(chǎn)品編號及規(guī)格：

RTD6125   10T（50μl）   300元

● 儲存條件：

  -20℃保存，開封后有效期12個(gè)月。

● 產(chǎn)品簡介：

本產(chǎn)品包含5種多肽和3種低分子量蛋白質(zhì)組成，分子量范圍為3.4kD-100kD?？梢杂脕砼袛?SDS-PAGE上多肽和小蛋白的分子量。  

● 使用說明：

第一次收到該產(chǎn)品，室溫融化后，徹底混勻，離心快甩將溶液完全收集到管底，根據(jù)需要適量分裝成小管，-20℃貯存，每次取一小管使用；本產(chǎn)品為即用型，融化后既能使用，不能95℃加熱處理。

一. 制膠：

I 配制分離膠

1．按照表一將不同體積的雙蒸水、40%PAA（19:1）、凝膠緩沖液和乙二醇加入到小燒杯中混合。

2．加入10%APS和TEMED，立即混勻5-10秒，以使溶液充分混勻。

3．在凝膠模具中迅速灌入適量分離膠溶液，然后在分離膠溶液上輕輕覆蓋一層1-3 cm的水層，使凝膠表面保持平整。

4．靜置30-60分鐘，待分離膠和水層之間出現(xiàn)一個(gè)清晰的界面表示凝膠已聚合。

表一  （一塊0.75mm mini膠用量）

注：如非必須，不要使用1.0mm和1.5mm的凝膠，盡量使用厚度0.75mm的凝膠，這樣會減少電泳后染色和脫色的時(shí)間。

II 配制濃縮膠

去除覆蓋在分離膠上的水層，用濾紙將殘留的水吸去。

1．按照表一將不同體積的雙蒸水、40%PAA（29:1）和凝膠緩沖液加入到小燒杯中混合。

2．加入10%過硫酸銨和TEMED，立即混勻5-10秒，以使溶液充分混勻。

3．將梳子插入凝膠內(nèi)，避免產(chǎn)生氣泡。

4．靜置30-60分鐘裝待凝膠聚合

二. 電泳：

1. 取一管分裝好的Marker樣品，徹底融化，不要加熱處理，充分混勻后備用。

2. 電泳前，將10×Tris-Tricine-SDS電泳緩沖液（Cat No：CB010）用蒸餾水稀釋成1×緩沖液備用。將電泳槽的內(nèi)外槽加入1×緩沖液，輕柔拔出梳子，將Marker或蛋白樣品（樣品已經(jīng)過2×Tricine上樣緩沖液[Cat No：TP050]處理）加入點(diǎn)樣孔，根據(jù)表二條件進(jìn)行電泳，至藍(lán)色指示前沿至分離膠3/4位置時(shí)即可停止電泳。整個(gè)電泳過程大約需要2-3個(gè)小時(shí)。注：如果電泳時(shí)間過短，染色時(shí)，指示前沿容易遮擋小于5kd的小肽。

表二 多肽電泳條件

三. 染色

根據(jù)常規(guī)實(shí)驗(yàn)步驟，用配方5和6（表三）進(jìn)行染色和觀察。如果使用配方5進(jìn)行染色時(shí)效果不好或考慮其毒性，請選擇本公司的FastBlue蛋白染色液（Cat No：RTD6202），該產(chǎn)品能在5分鐘之內(nèi)完成蛋白的染色，不至于小肽在染色和脫色中從凝膠中脫離，是常規(guī)染色液的理想替代品。

表三 小分子蛋白質(zhì)SDS-PAGE電泳試劑配制

使用蛋白Marker產(chǎn)品發(fā)表部分文章列表：

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control 50 (2015) 896e900

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：http://dx.doi.org/10.1016/j.foodcont.2014.10.040

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：https://doi.org/10.1016/j.foodcont.2017.05.030

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

Marker: RTD6127，超低分子量蛋白Marker IV（3.3-45 kD）

Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

Institution：College of Biological Science and Biotechnology, Beijing Forestry University

Paper link：https://link.springer.com/article/10.1007/s00217-017-2958-3

5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker （1.2-45 kD）

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019，613-626

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110，彩虹預(yù)染超低分子量蛋白（1.2-45 kD）

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

Institution：Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper link：https://doi.org/10.1093/plcell/koab046

7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110，彩虹預(yù)染超低分子量蛋白（1.2-45 kD）

Journal: Analytical Methods. 2021,13, 2804-2811

Institution：Department of Chemistry, Fudan University

Paper link：https://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

Marker: RTD6142 高分子量非變性電泳蛋白質(zhì)Marker II（45-669 kD）

Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

Institution：College of Veterinary Medicine, Hunan Agricultural University,

Paper link：https://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

Marker: RTD6109 高分子量蛋白質(zhì)Marker（43-200 kD）

Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

Institution：College of Food Science and Technology, Hebei Normal University of Science and Technology

Paper link：https://link.springer.com/article/10.1134/S0003683810060116

10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

Author: Dong Liu

Marker: RTD6103 低分子量蛋白質(zhì)Marker II（14.4-116 kD）

Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

Institution：Department of Food Processing Technology, Anqing Vocational & Technical College

Paper link：https://doi.org/10.1111/1471-0307.12395

11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

Marker: Protein Marker

Journal: Biomedical Materials, 2022 Jan 24;17(2)

Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Paper link：https://pubmed.ncbi.nlm.nih.gov/34996052/